Introduction to the Bradford Assay eForm

"What is this Bradford Assay eForm?" you might be asking. It is an online data processing application that serves as a template for an experimenter or researcher to submit their protein assay data via a web form. The data is subsequently processed in real-time to produce a report of the Bradford protein determination assay, to determine and apply statistically significant analysis of that data, to evaluate that data such that it produces the appropriate calculations to derive protein concentration in experimental unknowns, and to produce final reports in various formats to meet the needs of the lab or institution. The data processing template is reusable, requires no end-user maintenance, and is modifiable such that customized output reflects the requirements of the investigator. It's simple. It's quick. And it's complete.

No software installation required. Just subscribe and use.

We have updated the site, but the data processing functions operate as before without modification. If you need a brief refresher on the Bradford assay, then by all means - review it.

If you need direct access to data processing, login to the Bradford Assay eForm. Otherwise, please continue reviewing the Bradford assay process and procedure.

Sample eForm Summary - the Components

The experimental summary report

The first part of your report summarizes your input in a table for quick reference. When you initiate your experiment, it is important that all pertinent data get recorded for both effective implementation of the scientific method and for efficient historical archiving of that data. Consistency of that archive aids in future comparisons between experiments as well as facilitate convenient recall of the information.

The purpose of the initial data block is to record the who, what, and when of any particular assay. Further, other noteworthy observations should be recorded that might help troubleshoot the experiment should something appear irregular; conversely, the noted observations serve to confirm that the experiment was performed properly and normal results ensued. Whatever the outcome of any given assay, it is vital that good records be taken - without them, the experiment will not serve as the complete record of physical observation.

Some of the features that we have found useful for producing a complete report are:

• Experiment Label
• Name of Researcher
• Name of Primary Investigator
• Experiment Date
• Experiment Time
• Project ID
• Experiment Series
• Experiment Number
• Project Notes
• Protein Standard Details
• Spectrophotometer Specifications
• Customized Barcode

The standard curve report

The second part of your report determines and graphs a regression curve for your standards.

Once the experimental background data has been entered into the eForm, you will proceed to enter standard curve specimen labels and values in preparation for the standard curve derivation. Upon data submission the eForm will derive the standard curve equation and report statistics and notes indicating the quality and validity of your standard samples.

Further, it will convert the data according to your preferences prior to formatting and tabulating. Some useful modifiers:

• Sample Units
• Conversion Factor
• Significant Figures
• Minimal Acceptable R²
• Sample Count

The eForm will also include the appropriately useful statistics:

• Derived Equation
• Intercept
• Slope
• R²
• Σ
• Durbin-Watson Test
• T-stat (intercept)
• T-stat (slope)
• Internal self-check of preset parameters

Next, the eForm will plot the results in a customizable format via different options:

• Average Curve Data Points
• All Curve Data Points
• Point-to-Point Curve
• Smoothed Curve
• Regression Line
• Magnified Inset for lowest concentrations

The experimental unknowns report

The third part of your report processes your input of experimental unknowns, tabulates and graphs the output, and provides options to print the report or download it in a database friendly format. You may also email your experimental data report should that be desired.

The currently available formats and options for final output of the Bradford Assay eForm are the following:

• Present labelled experimental unknowns (up to triplicate), average values, and the derived spectrophotgraphic absorbance with appropriate significant figures
• Present tabulated results in an easily readable bargraph
• Identify minimum, average, and maximum values from experimental dataset
• Print the entire report directly from your browser
• Present/Save the data as either a comma-delimited, tab-delimited, or Microsoft Excel formatted spreadsheet
• Present/Save the data as a MS Word document
• Present/Save the data as a Portable Document Format (PDF)
• Save/Submit the data as an email